Coding

Part:BBa_K184000:Design

Designed by: Jennifer Lei   Group: iGEM09_Bay_Area_RSI   (2009-10-14)


Silintaphin-1 + gs linker partA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 569
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 460
    Illegal SapI.rc site found at 736
    Illegal SapI.rc site found at 892


Design Notes

Synthetic gene assembly of Silintaphin-1 was created by splitting the Silintaphin-1 gene into (2) separate parts because of high GC content and repeat sequences introduced by the (GS4)6 linker.


Source

Silintaphin-1 was created by synthetic gene synthesis with silent mutations to delete biobrick cloning sites in the endogeneous gene sequence. The gene was also codon optimised for human and e.coli expression. The Silintaphin-1 gene was derived from the silicanaceous sponge, Suberites domuncula.

References